The Rainey Lab
Dal logo Department of Biochemistry & Molecular Biology

Halifax, Nova Scotia Canada
Refereed journal articles (trainees listed in bold)

Under review:

57. Kenward, K., Shin, K. and Rainey, J.K. (2017) Fluorotryptophan mixtures as a tool to expand versatility of 19F protein NMR spectroscopy. Chemistry - A European Journal. Under review.

56. Shin, K., Landsman, M. Pelletier S., Alamri, B., Anini, Y. and Rainey, J.K. (2017) Proapelin is processed extracellularly in a cell-line dependent manner with clear modulation by proprotein convertases. Peptides. Under review.

In Press:

55. Shin, K., Kenward, K. and Rainey, J.K. (2017) Apelinergic system structure and function. Comprehensive Physiology. (Peer-reviewed invited Overview Article.)


Published.


54. Shin, K.Sarker, M., Huang, S.K. and Rainey, J.K. (2017) Apelin conformational and binding equilibria upon micelle interaction primarily depend on membrane-mimetic headgroup. Scientific Reports. 7: 15433. Link to article.

53. Xu, L., Lefèvre, T., Orrell, K.E., Meng, Q., Auger, M., Liu, X-Q. and Rainey, J.K. (2017) Structural and mechanical roles for the C-terminal non-repetitive domain become apparent in recombinant spider aciniform silk. Biomacromolecules. 18: 3678-3686. Link to article.
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52. Das, D., Shin, K.,  Rainey, J.K. and Fliegel L. (2017) Transmembrane segment XI of the Na+/H+ antiporter of S. pombe is a critical part of the ion translocation pore.  Scientific Reports. 7: 12793. Link to article.

51. Shin, K., Chapman, N.A., Sarker, M., Kenward, K., Huang, S.K., Weatherbee-Martin, N., Pandey, A., Dupré, D.J. and Rainey, J.K. (2017) Bioactivity of the putative apelin proprotein expands the repertoire of apelin receptor ligands. Biochimica et Biophysica Acta - General Subjects. 1861: 1901-1912. Link to article.

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50. Langelaan, D.N., Pandey, A., Sarker, M. and Rainey, J.K. (2017) Preserved transmembrane segment topology, structure, and dynamics in disparate micellar environments. The Journal of Physical Chemistry Letters. 8: 2381-2386. Link to article.

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49. Patterson, R.E., Weatherbee-Martin, N. and Rainey, J.K. (2017) Pyrene-apelin conjugation modulates fluorophore- and peptide-micelle interactions. The Journal of Physical Chemistry B. 121: 4768-4777. Link to article.

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48. Huang, S.K.+, Shin, K.+, Sarker, M. and Rainey, J.K. (+contributed equally; 2017)  Apela exhibits isoform- and headgroup-dependent modulation of micelle binding, peptide conformation and dynamics. Biochimica et Biophysica Acta - Biomembranes. 1859: 767-778. Permanent link to article. Free download link.

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47. Zhong, X.Z., Zou, Y., Sun, X., Dong, G., Cao, Q., Pandey, A., Rainey, J.K., Zhu, X. and Dong, X-P. (2017) Inhibition of TRPML1 by lysosomal adenosine involved in severe combined immunodeficiency diseases. Journal of Biological Chemistry. 292: 3445-3455. Link to article.

46. Young, B.M., Nguyen, E., Chedraw, M.A.J., Rainey, J.K. and Dupré, D.J. (2017) Differential contribution of transmembrane domains IV, V, VI, and VII to human angiotensin II type 1 receptor homomer formation. Journal of Biological Chemistry. 292: 3341-3350. Link to article.

45. Pandey, A., Shin, K., Patterson, R.E., Liu, X-Q. and Rainey, J.K. (2016) Current strategies for protein production and purification enabling membrane protein structural biology. Biochemistry and Cell Biology. 94: 507-527. Link to article.

44. Tremblay, M-L., Xu, L., Sarker, M., Liu, X-Q. and Rainey, J.K. (2016) Characterizing aciniform silk repetitive domain backbone dynamics and hydrodynamic modularity. International Journal of Molecular Sciences. 17: 1305. Link to article. (Invited peer reviewed submission for Special Issue on "Silk-Based Materials: From Production to Characterization").

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43. Weatherbee-Martin, N., Xu, L., Hupe, A., Kreplak, L., Fudge, D.S., Liu, X-Q. and Rainey, J.K. (2016) Identification of wet-spinning and post-spin stretching methods amenable to recombinant spider aciniform silk. Biomacromolecules. 17: 2737-2746. Link to article.

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42. Sarker, M.+, Orrell, K.E.+, Xu, L., Tremblay, M-L., Bak, J.J., Liu, X-Q. and Rainey, J.K. (+contributed equally; 2016) Tracking transitions in spider wrapping silk conformation and dynamics by 19F nuclear magnetic resonance spectroscopy. Biochemistry. 55: 3048-3059. Link to article.

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41. Eftaiha, A.F., Tremblay, M-L., Rainey, J.K. and Paige, M.F. (2015) The effect of perfluorooctadecanoic acid on a model phosphatidylcholine-peptide pulmonary lung surfactant mixture. Journal of Fluorine Chemistry. 177: 55-61. Link to article.

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40. Tremblay, M-L.+, Xu, L.+, Lef�vre, T., Sarker, M., Orrell, K.E., Leclerc, J., Meng, Q., Pezolet, M., Auger, M., Liu, X-Q. and Rainey, J.K. (+ contributed equally; 2015) Spider wrapping silk fiber architecture arising from its modular soluble protein precursor. Scientific Reports. 5: 11502. Link to article.

39. Read, J.+, Clancy, E.K.+, Sarker, M., de Antueno, R., Langelaan, D.N., Parmar, H.B., Shin, K., Rainey, J.K. and Duncan, R. (+contributed equally; 2015) Reovirus FAST proteins drive pore formation and syncytiogenesis using a novel helix-loop-helix fusion-inducing lipid packing sensor. PLoS Pathogens. 11: e1004962. Link to article.

38. Clattenburg, L., Wigerius, M., Qi, J., Rainey, J.K., Rourke, J.L., Muruganandan, S., Sinal, C.J. and Fawcett, J.P. (2015) NOS1AP functionally associates with YAP to regulate Hippo signaling. Molecular and Cellular Biology. 35: 2265-2277. Link to article.

37. Sarker, M., Fraser, R.E., Lumsden, M.D., Anderson, D.J. and Rainey, J.K. (2015) Characterization of variant soft nanoparticle structure and morphology in solution by NMR spectroscopy. The Journal of Physical Chemistry C. 119: 7461-7471. Link to article.

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36. Key, T., Sarker, M., de Antueno, R., Rainey, J.K. and Duncan, R. (2015) The p10 FAST protein fusion peptide functions as a cystine noose to induce cholesterol-dependent liposome fusion without liposome tubulation. Biochmica et Biophysica Acta - Biomembranes. 1848: 408-416. Link to article.

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35.Chapman, N.A., Dupr�, D.J. and Rainey, J.K. (2014) The apelin receptor: Physiology, pathology, cell signalling, and ligand modulation of a peptide-activated class A GPCR. Biochemistry and Cell Biology. 92: 431-440. Link to article.

34. Pandey, A., Sarker, M., Liu, X-Q. and Rainey, J.K. (2014) Small expression tags enhance bacterial expression of the first three transmembrane segments of the apelin receptor. Biochemistry and Cell Biology. 92: 269-278. Link to article.

33. Xu, L., Tremblay, M-L., Orrell, K.E., Leclerc, J., Meng, Q., Liu, X-Q. and Rainey, J.K. (2013) Nanoparticle self-assembly by a highly stable recombinant spider wrapping silk protein subunit. FEBS Letters. 587: 3273-3280. Link to article.

32. Shin, K., Pandey, A., Liu, X-Q., Anini, Y. and Rainey, J.K. (2013) Preferential apelin-13 production by the proprotein convertase PCSK3 is implicated in obesity. FEBS Open Biology. 3: 328-333. Link to article (open access).

31. Kehoe, S., Tremblay, M-L., Coughlan, A., Towler, M.R., Rainey, J.K., Abraham, R.J. and Boyd, D. (2013) Preliminary Investigation of the Dissolution Behavior, Cytocompatibility, Effects of Fibrinogen Conformation and Platelet Activation for Radiopaque Embolic Particles. Journal of Functional Biomaterials. 4: 89-113. Link to article (open access).

30. Langelaan, D.N., Reddy, T., Banks, A.W., Dellaire, G., Dupr�, D.J. and Rainey, J.K. (2013) Structural features of the apelin receptor N-terminal tail and first transmembrane segment implicated in ligand binding and receptor trafficking. Biochimica et Biophysica Acta - Biomembranes. 1828: 1471-1483. Link to article (freely available). PDB entry (2LOU). BMRB entry (18225).

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29. Ramu, T., Prasad, M., Connors, E., Mishra, A., Thomassin, J., Leblanc, J., Rainey, J.K. and Thomas, N. (2013) A novel C-terminal region within the multicargo type III secretion chaperone CesT contributes to effector secretion. Journal of Bacteriology195: 740-756. Pubmed link.

28. Xu, L., Rainey, J.K., Meng, Q. and Liu, X-Q. (2012) Recombinant minimalist spider wrapping silk proteins capable of native-like fiber formation. PLOS ONE. 7: e50227. Link to article.

27. Xu, L.+, Tremblay, M-L.+, Meng, Q., Liu, X-Q. and Rainey, J.K. (+contributed equally; 2012) 1H, 13C and 15N NMR assignments of the aciniform spidroin (AcSp1) repetitive domain of Argiope trifasciata wrapping silk. Biomolecular NMR Assignments. 6: 147-151. Pubmed link.

26. Reddy, T. and Rainey, J.K. (2012) The multifaceted substrate capture scheme of a rhomboid protease. The Journal of Physical Chemistry B. 116: 8942-8954. Link to article.

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25. Langelaan, D.N.+, Ngweniform, P.+ and Rainey, J.K. (+contributed equally; 2011) Biophysical characterization of G-protein coupled receptor-peptide ligand binding. Biochemistry and Cell Biology. 89: 98-105. Link to article.

24. Langelaan, D.N., Wieczorek, M., Blouin, C. and Rainey, J.K. (2010) Improved helix and kink characterization in membrane proteins allows evaluation of kink sequence predictors. Journal of Chemical Information and Modeling. 50: 2213-2220. Link to article; Download link for non-subscribers; Link to MC-HELAN web interface.

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23. Tremblay, M-L., Banks, A.W. and Rainey, J.K. (2010) The predictive accuracy of secondary chemical shifts is more affected by protein secondary structure than solvent environment. Journal of Biomolecular NMR46: 257-270. Link to article. Link to CS-CHEMeleon web interface.

22. Langelaan, D.N. and Rainey, J.K. (2010) Membrane catalysis of peptide-receptor binding. Biochemistry and Cell Biology. 88: 203-210. Link to article.

21. Reddy, T. and Rainey, J.K. (2010) Interpretation of biomolecular NMR spin relaxation parameters. Biochemistry and Cell Biology. 88: 131-142. Link to article. Link to relaxation calculation web interface.

20. Reddy, T.Li, X., Fliegel, L., Sykes, B.D. and Rainey, J.K. (2010) Correlating structure, dynamics, and function in transmembrane segment VII of the Na+/H+ exchanger isoform 1. Biochimica et Biophysica Acta - Biomembranes. 1798: 94-104. Pubmed link; link to article. (Invited peer reviewed submission.) 

19. Langelaan, D.N. and Rainey, J.K. (2009) Headgroup-dependent membrane catalysis of apelin-receptor interactions is likely. The Journal of Physical Chemistry B. 113: 10465-10471.  Abstract/Download (open access availability); PDB file & chemical shifts for apelin-17 bound to SDS micelles: BMRB entry 20082chemical shifts for apelin-17 in presence of DPC
micelles: BMRB entry 16275.

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18. Langelaan, D.N., Bebbington, E.M., Reddy, T. and Rainey, J.K. (2009) Structural insight into G-protein coupled receptor binding by apelin. Biochemistry. 48: 537-548. Abstract/Download; Download link for non-subscribers; BMRB entries with chemical shifts & PDB files: Apelin-17 at 35�C (20029)Apelin-17 at 5�C - major conformer (20030)Apelin-17 at 5�C - minor conformer (20031).

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17. Reddy, T.+Ding, J.+, Li, X., Sykes, B.D., Rainey, J.K.and Fliegel, L. (+contributed equally; 2008) Structural and functional characterization of transmembrane segment IX of the NHE1 isoform of the Na+/H+ exchanger. Journal of Biological Chemistry. 283: 22018-22030. Abstract/Download; PDF (840 kB); PDB entry (2KC3). BMRB entry (15747).

Covering work by Dr. Rainey prior to arrival at Dalhousie:

16. Rainey, J.K., DeVries, J.S., and Sykes, B.D. (2007) Estimation and measurement of flat or solenoidal coil inductance for radiofrequency NMR coil design. Journal of Magnetic Resonance. 187: 27-37. Abstract/Download; PDF (544 kB); Supplementary Data; Excel spreadsheet for coil calculation.

15. Slepkov, E.R., Rainey, J.K., Sykes, B.D. and Fliegel, L. (2007) Structural and functional analysis of the Na+/H+ exchanger. Biochemical Journal. 401: 623-633. Abstract/download; PDF (620 kB).

14. Rainey, J.K., Fliegel, L. and Sykes, B.D. (2006) Strategies for dealing with conformational sampling in structural calculations of flexible or kinked transmembrane peptides. Biochemistry and Cell Biology. 84: 918-929. Abstract/download; PDF (932 kB).

13.  Ding, J.+, Rainey, J.K.+, Xu, C., Sykes, B.D. and Fliegel, L. (+contributed equally; 2006) Structural and functional characterization of transmembrane segment VII of the Na+/H+ exchanger isoform 1. Journal of Biological Chemistry. 281: 29817-29829. Abstract/download; PDF (740 kB); Supplemental data. PDB entry (2HTG). BMRB entry (7245).

12. Baryshnikova, O.K., Rainey, J.K., and Sykes, B.D. (2005) Nuclear magnetic resonance studies of CXC chemokine receptor 4 allosteric peptide agonists in solution. Journal of Peptide Research. 66 (s1): 12-21. Abstract/download (free).

11. Rainey, J.K. and Sykes, B.D. (2005) Optimizing oriented planar supported lipid samples for solid-state protein NMR. Biophysical Journal, 89: 2792-2805. Abstract/download; PDF (344 kB); Supplementary information.

10. Rainey, J.K., DeVries, J.S., and Sykes, B.D. (2005) A rotatable flat-coil for static solid-state nuclear magnetic resonance spectroscopy. Review of Scientific Instruments, 76: 086102. Abstract/download; PDF (252 kB).

9. Slepkov, E.R., Rainey, J.K., Li, X., Liu, Y., Cheng, F.J., Lindhout, D.A., Sykes, B.D. and Fliegel, L. (2005) Structural and functional characterization of transmembrane segment IV of the NHE1 isoform of the Na+/H+ exchanger. Journal of Biological Chemistry. 280: 17863-17872. Abstract/download; PDF (560 kB); PDB entry (1Y4E). BMRB entry (6446).

8. Rainey, J.K. and Goh, M.C. (2004) An interactive triple-helical collagen builder. Bioinformatics. 20: 2458-2459. Paper download (no subscription required). Supplementary Information and Tcl/Tk Script.

7. Rainey, J.K. and Goh, M.C. (2004) Statistically based reduced representation of amino acid side-chains. Journal of Chemical Information and Computer Sciences. 44: 817-830. PDF (521 kB); Supporting information.

6. Rainey, J.K., Wen, C.K., and Goh, M.C. (2002) Hierarchical assembly and the onset of banding in fibrous long spacing collagen revealed by atomic force microscopy. Matrix Biology. 21: 647-661. PDF (2.4 MB)

5. Rainey, J.K. and Goh, M.C. (2002) A statistically derived parameterization for the collagen triple-helix. Protein Science. 11: 2748-2754. PDF (136 kB - includes Correction published in 13: 2276) and Supplementary material

4. Rainey, J.K. and Goh, M.C. (2002) Parallel atomic force microscopy and NMR spectroscopy to investigate self-assembled protein-nucleotide aggregates. The Journal of Physical Chemistry B. 106: 5553-5560. PDF (280 kB)

3. Paige, M.F., Rainey, J.K., and Goh, M.C. (2001) A study of fibrous long spacing collagen ultrastructure and assembly by atomic force microscopy. Micron. 32: 341-53. PDF (1.8 MB)

2. Mayo, P., Poirier, M., Rainey, J., and Tam, W. (1999) Effect of a remote substituent on regioselectivity in oxymercuration of unsymmetrically substituted norborenes. Tetrahedron Letters. 40: 7727-30. PDF (200 kB)

1. Paige, M.F., Rainey, J.K., and Goh, M.C. (1998) Fibrous long spacing collagen ultrastructure elucidated by atomic force microscopy. Biophysical Journal. 74: 3211-16. PDF (248 kB)


Note: these downloads are provided for personal research/study use for non-commercial purposes - if you wish to make commercial use of an article itself or its content, please contact the copyright holder.

Conference Presentations (primary presenter underlined)

122. Reda, A.M., Tremblay, M-L., Rainey, J.K. and McLeod, R.S. (2014) Structural Characterization of 20 kDa Fragments of Apolipoprotein B100. Arteriosclerosis, Thrombosis and Vascular Biology 2014, Toronto, ON. (Poster.)

121. Chapman, N., Holstead, R.G., Dupr�, D.J. and Rainey, J.K. (2014) Probing GPCR activation: Functional analysis of the N-terminus and extracellular loops of the apelin receptor. 57th Annual Meeting of the Canadian Society of Molecular Biosciences, Banff, AB. (Poster.) CSMB Student Travel Award to N. Chapman.

120. Langelaan, D.N., Sarker, M., Pandey, A. and Rainey, J.K. (2014) Surprising structural and dynamic invariance of a transmembrane domain in four disparate membrane-mimetics. 57th Annual Meeting of the Canadian Society of Molecular Biosciences, Banff, AB. (Poster.)

119. Pandey, A., Sarker, M., Scott, E.M., Liu, X-Q. and Rainey, J.K. (2014) Dividing to conquer: Facilitating recombinant expression and NMR spectroscopy. 57th Annual Meeting of the Canadian Society of Molecular Biosciences, Banff, AB. (Poster.)

118. Langelaan, D.N., Pandey, A., Sarker, M., Tremblay, M-L., Xu, L., Reddy, T., Banks, A.W., Liu, X-Q. and Rainey, J.K. (2014) Contextualizing membrane protein structure despite the �divide and conquer� approach. Overcoming Barriers to Membrane Protein Structure Satellite Symposium, 57th Annual Meeting of the Canadian Society of Molecular Biosciences, Banff, AB. (Invited Oral Presentation.)

117. Tremblay, M-L., Xu, L., Sarker, M., Lef�vre, T., Leclerc, J. P�zolet, M., Auger, M., Meng, Q., Liu, X-Q., and Rainey, J.K. (2014) Spider Wrapping Silk: A Structural Perspective From Solution State to Fibrillogenesis. 55th Experimental Nuclear Magnetic Resonance Conference (ENC), Boston, MA. (Oral presentation and poster.) ENC Student Travel Stipend to MLT.

116. Pandey, A., Sarker, M., Liu, X-Q. and Rainey, J.K. (2013) Structural characterization of the apelin receptor. GPCR Workshop 2013, Maui, HI (Poster.) DMRF Weldon Travel Award to A. Pandey.

115. Pandey, A., Sarker, M., Liu, X-Q. and Rainey, J.K. (2013) Structural characterization of the apelin receptor. MOOT XXVI NMR Symposium, Kingston, ON (Poster.)

114. Sarker, M., Tremblay, M-L., Xu, L., Liu, X-Q. and Rainey, J.K. (2013) Insight into Spider Wrapping Silk Fiber Formation by Diffusion NMR. MOOT XXVI NMR Symposium, Kingston, ON (Poster.)

113. Shin, K., Pandey, A., Anini, Y. and Rainey, J.K. (2013) Structural characterization of proapelin processing. MOOT XXVI NMR Symposium, Kingston, ON (Poster.)

112. Tremblay, M-L., Xu, L., Sarker, M., Meng, Q., Liu, X-Q. and Rainey, J.K. (2013) Using Split Inteins to Reveal the Striking Modularity of Spider Wrapping Silk in Solution. MOOT XXVI NMR Symposium, Kingston, ON (Oral presentation.)

111. Chapman, N., Dupr�, D.J. and Rainey, J.K. (2013) Structural characterization of the extracellular region of the apelin receptor and its interaction with apelin. 96th Canadian Society for Chemistry Conference & Exhibition, Qu�bec, QC. (Poster.)

110. Pandey, A., Liu, X-Q. and Rainey, J.K. (2013) Structural characterization of the apelin receptor. 96th Canadian Society for Chemistry Conference & Exhibition, Qu�bec, QC. (Poster.)

109. Sarker, M., Speckert, M. and Rainey, J.K. (2013) Head-group dependent interactions of the apelin peptide hormones with phospholipid bicelles. 96th Canadian Society for Chemistry Conference & Exhibition, Qu�bec, QC. (Poster.)

108. Shin, K., Pandey, A., Anini, Y. and Rainey, J.K. (2013) The bioactive peptide apelin is activated to a specific isoform by proprotein convertase subtilin kexin-3. 96th Canadian Society for Chemistry Conference & Exhibition, Qu�bec, QC. (Oral presentation.)

107. Tremblay, M-L., Xu, L., Sarker, M., Meng, Q., Liu, X-Q. and Rainey, J.K. (2013) The exquisite modularity of spider wrapping silk provides insight into fibrillogenesis. 96th Canadian Society for Chemistry Conference & Exhibition, Qu�bec, QC. (Oral presentation.) Industrial Chemistry Division Presentation Award to M-L. Tremblay.

106. Xu, L., Tremblay, M-L., Meng, Q., Liu, X-Q. and Rainey, J.K. (2013) Recombinant spider silk assembly from soluble protein to solid fiber. 96th Canadian Society for Chemistry Conference & Exhibition, Qu�bec, QC. (Poster.)

105. Xu, L., Tremblay, M-L., Sarker, M., Meng, Q., Liu, X-Q. and Rainey, J.K. (2013) Using split inteins to reveal the striking modularity of spider wrapping silk. 96th Canadian Society for Chemistry Conference & Exhibition, Qu�bec, QC. (Oral presentation.)

104. Langelaan, D.N. and Rainey, J.K. (2012) Striking structural invariance of a transmembrane domain in four disparate membrane mimetics. 25th NMR MOOT Symposium, Qu�bec, QC. (Poster.)

103. Pandey, A., Liu, X-Q. and Rainey, JK. (2012) Structural characterization of the apelin receptor. 25th NMR MOOT Symposium, Qu�bec, QC. (Poster.)

102. Sarker, M., Fraser, R.E., Anderson, D.J. and Rainey, JK. (2012) Nanoparticles for improved pesticide delivery: conformation and loading mechanism revealed by NMR spectroscopy. 25th NMR MOOT Symposium, Qu�bec, QC. (Poster.)

101. Shin, K., Pandey, A., Anini, Y. and Rainey, JK. (2012) Proapelin activation into apelin-13 by proprotein convertase subtilin kexin-3. 25th NMR MOOT Symposium, Qu�bec, QC. (Poster.)

100. Tremblay, M-L., Xu, L., Werner-Zwanziger, U., Meng, Q., Liu, X-Q. and Rainey, JK. (2012) Solution-state NMR structure of recombinant aciniform spider silk. 25th NMR MOOT Symposium, Qu�bec, QC. (Oral presentation.) Wilmad Labglass Student Travel Award to MLT.

99. Xu, L., Tremblay, M-L., Werner-Zwanziger, U., Meng, Q., Liu, X-Q. and Rainey, JK. (2012) Segmental isotopic labeling of recombinant spider wrapping silk AcSp1 by protein trans-splicing. 25th NMR MOOT Symposium, Qu�bec, QC. (Poster.)

98.  Langelaan, D.N. and Rainey, J.K. (2012) Surprising structural invariance of a transmembrane domain in four disparate membrane mimetics. XXVth International Conference on Magnetic Resonance of Biological Systems. Lyon, France (Poster.)

97.  Sarker, M., Key, T., Langelaan, D., Werner-Zwanziger, U., Rainey, J. and Duncan, R. (2012) Critical role and features of a transmembrane Arced Helix (ArcH) in cell-cell membrane fusion. XXVth International Conference on Magnetic Resonance of Biological Systems. Lyon, France (Poster.)

96. Tremblay, M-L., Xu, L., Werner-Zwanziger, U., Liu, X-Q and Rainey, J.K. (2012) Recombinant aciniform spidroin spider silk characterization by solution- and solid-state NMR. XXVth International Conference on Magnetic Resonance of Biological Systems. Lyon, France (Oral presentation and poster.)

95. Shin, K., Pandey, A. Anini, Y. and Rainey, J.K. (2012) Differential apelin isoform production and involvement in obesity. REGPEP 2012 - 19th International Symposium on Regulatory Peptides, Copenhagen, Denmark. (Oral presentation.)

94. Tremblay, M-L., Xu, L., Meng, Q., Liu, X-Q. and Rainey, J.K. (2012) Characteristics of spider wrapping silk and its potential as a biomaterial. 39th Annual Microscopical Society of Canada Meeting, Halifax, NS. (Oral presentation.)

93. Banks, A.W., Ngweniform, P., Wieczorek, M., Bebbington, E.M., Langelaan, D.N., Reddy, T. and Rainey, J.K. (2012) Computational underpinning of electronic circular dichroism and nuclear magnetic resonance spectroscopy of polypeptides. 95th Canadian Society for Chemistry Conference & Exhibition, Calgary, AB. (Invited Oral Presentation in "Theoretical Approaches to Molecular Spectroscopy" session.)

92. Sarker, M., Fraser, R., Anderson, D. and Rainey, J.K. (2012) Nanoparticle conformation and active ingredient loading revealed by NMR spectroscopy. 11th Chemical Biophysics Symposium, Toronto, ON. (Poster)

91. Langelaan, D.N. and Rainey, J.K. (2012) Unchanged transmembrane segment kink and topology despite variation in membrane-mimetic environment. 56th Biophysical Society Meeting, San Diego, CA. (Poster)

90. Ngweniform, P., Langelaan, D.N., Chapman, N. and Rainey, J.K. (2012) Dissecting the roles of the extracellular regions in apelin peptide-receptor binding. 56th Biophysical Society Meeting, San Diego, CA. (Poster) Biophysical Society Committee for Professional Opportunities for Women Travel Award to PN.

89. Pandey, A. and Rainey, J.K. (2012) Structure and dynamics provides direct insight into proapelin processing. 56th Biophysical Society Meeting, San Diego, CA. (Poster)

88. Tremblay, M-L., Xu, L., Meng, Q., Liu, X-Q. and Rainey, J.K. (2012) Structural evidence for the micellar model of spider silk fibrillogenesis. 56th Biophysical Society Meeting, San Diego, CA. (Poster)

87. Ngweniform, P. and Rainey, J.K. (2011) Evidence of binding between the first extracellular loop of the apelin receptor, and its cognate ligand in lipid environments. Canadian Cancer Research Conference, Toronto, ON. (Poster)

86. Ngweniform, P. and Rainey, J.K. (2011) Evidence of binding between the first extracellular loop of the apelin receptor, and its cognate ligand in lipid environments. Cancer Research Symposium, Halifax, NS. (Poster)

85. Langelaan, D.N., Banks, A.W., Dellaire, G., Dupr�, D.J. and Rainey, J.K. (2011) Structural and functional correlation of the N-terminus and first transmembrane segment of the apelin receptor. 24th NMR MOOT Symposium, Toronto, ON. (Poster)

84. Sarker, M., Werner-Zwanziger, U. and Rainey, J.K. (2011) Solid-state NMR investigation of bicelle structural organization and peptide interactions. 24th NMR MOOT Symposium, Toronto, ON. (Poster)

83. Tremblay, M-L., Xu, L., Liu, X-Q. and Rainey, J.K. (2011) NMR solution structure of the monomeric aciniform spider silk and characterization by AFM. 24th NMR MOOT Symposium, Toronto, ON. (Poster) Suraj Manrao Poster Prize to MLT.

82. Banks, A.W.*, Langelaan, D.N. and Rainey, J.K. (2011) Developing methods for hybrid NMR spectroscopy and homology model based structures of membrane proteins. 94th Canadian Society for Chemistry Conference & Exhibition, Montr�al, QC. (Poster)

81. Langelaan, D.N. and Rainey, J.K. (2011) Effects of micelle environment upon structure, dynamics and topology of the apelin receptor N-terminus and first transmembrane domain. 94th Canadian Society for Chemistry Conference & Exhibition, Montr�al, QC. (Invited oral presentation in Solution NMR: Biomolecular Structure, Dynamics, and Function session).

80. Langelaan, D.N. and Rainey, J.K. (2011) Structural and functional characterization of the N-terminal tail and first transmembrane segment of the apelin receptor. 94th Canadian Society for Chemistry Conference & Exhibition, Montr�al, QC. (Poster)

79. Ngweniform, P. and Rainey, J.K. (2011) Conformation-induced binding of extracellular loops of the apelin receptor with the apelin ligand in lipid environments. 94th Canadian Society for Chemistry Conference & Exhibition, Montr�al, QC. (Oral presentation)

78. Sarker, M. and Rainey, J.K. (2011) Solid-State NMR Investigation of Bicelle Lipid Distribution and Peptide Interactions. 94th Canadian Society for Chemistry Conference & Exhibition, Montr�al, QC. (Poster)

77. Tremblay, M-L., Xu, L., Liu, P.X-Q. and Rainey, J.K. (2011) Recombinant aciniform spidroin spider silk monomer and fiber characterization by NMR and AFM. 94th Canadian Society for Chemistry Conference & Exhibition, Montr�al, QC. (Poster)

76. Langelaan, D.N. and Rainey, J.K. (2011) Structure and topology of the N-terminal tail and first transmembrane segment of the apelin receptor in three different micelle types. 10th Chemical Biophysics Symposium, Toronto, ON. (Oral presentation)

75. Ngweniform, P. and Rainey, J.K. (2011) Conformation-induced binding of extracellular loops of the apelin receptor with the apelin ligand in lipid environments. 10th Chemical Biophysics Symposium, Toronto, ON. (Poster)

74. Sarker, M., Key, T., Rainey, J.K. and Duncan, R. (2011) A viral membrane fusion protein transmembrane domain containing a non-canonical interfacial helix and a central helical kink. 10th Chemical Biophysics Symposium, Toronto, ON. (Poster)

73. Tremblay, M-L., Xu, L., Liu, P.X-Q. and Rainey, J.K. (2011) NMR and AFM characterization of recombinant spider silk monomers and fibers. 10th Chemical Biophysics Symposium, Toronto, ON. (Poster)

72. Langelaan, D.N., Sarker, M. and Rainey, J.K. (2011) A structural comparison of the N-terminal segment of the apelin receptor in various membrane mimetics. 55th Biophysical Society Meeting,, Baltimore, MD. (Oral presentation)

71. Ngweniform, P. and Rainey, J.K. (2011) Probing binding of apelin to the extracellular loops of the apelin receptor (APJ) in lipid mimetic environments. 55th Biophysical Society Meeting,, Baltimore, MD. (Poster)

70. Tremblay, M-L., Xu, L., Liu, P.X-Q. and Rainey, J.K. (2011) Characterizing recombinant spider wrapping silk monomers and fibers by NMR and AFM. 55th Biophysical Society Meeting, Baltimore, MD. (Poster)

69. Ngweniform, P. and Rainey, J.K. (2010) Conformation-induced binding of extracellular loop 1 of the apelin receptor with apelin ligand in lipid environments. BHCRI Cancer Research Symposium, Halifax, NS.

68. Banks, A.W., Tremblay, M-L. and Rainey, J.K. (2010) CS-CHEMeleon: Predicting protein secondary structure in disparate environments using random coil chemical shifts. 23rd MOOT Mini NMR Symposium, Halifax, NS. (Poster)

67. Doyle, C.A. and Rainey, J.K. (2010) Diffusion ordered spectroscopy of the GPCR ligand apelin and mutant analogues. 23rd MOOT Mini NMR Symposium, Halifax, NS. (Poster)

66. Langelaan, D.N. and Rainey, J.K. (2010) Structural and functional characterization of the N-terminus and first transmembrane segment of the apelin receptor. 23rd MOOT Mini NMR Symposium, Halifax, NS. (Oral presentation)

65. Langelaan, D.N., Bebbington, E.M., Reddy, T. and Rainey, J.K. (2010) Evidence for membrane-catalyzed apelin-receptor binding. 23rd MOOT Mini NMR Symposium, Halifax, NS. (Poster)

64. Ngweniform, P. and Rainey, J.K. (2010) Biophysical characterization of the conformation induced binding of the first extracellular loop of the apelin receptor in lipid environment. 23rd MOOT Mini NMR Symposium, Halifax, NS. (Poster)

63. Reddy, T., Sykes, B.D. and Rainey, J.K. (2010) Probing the effects of slow-exchange conformational sampling upon dynamics determined by peptide backbone 15N-relaxation. 23rd MOOT Mini NMR Symposium, Halifax, NS. (Poster)

62. Tremblay, M-L., Xu, L., Liu, P.X-Q. and Rainey, J.K. (2010) From single proteins to fibers: spider silk structural studies. 23rd MOOT Mini NMR Symposium, Halifax, NS. (Poster) Suraj Manrao Student Poster Prize Winner.

61. Langelaan, D.N. and Rainey, J.K. (2010) Determining structure and apelin binding in the N-terminus and first transmembrane segment of APJ. REGPEP 2010 - 18th International Symposium on Regulatory Peptides, Belfast, UK. 1 of 4 abstracts selected for inclusion as a talk in first international Apelin mini-symposium.

60. Langelaan, D.N., Bebbington, E.M., Reddy, T. and Rainey, J.K. (2010) Structural studies of apelin yielding insight into function. REGPEP 2010 - 18th International Symposium on Regulatory Peptides, Belfast, UK. Poster.

59. Ngweniform, P. and Rainey, J.K. (2010) Fluorescence resonance energy transfer between apelin and its cognate G-protein coupled receptor in membrane mimetic environments. Terry Fox Research Institute 2nd Annual Scientific Meeting, Vancouver, BC. (Poster) Travel award to P. Ngweniform.

58.   Langelaan, D.N. and Rainey, J.K. (2010) MC-HELAN: A Monte Carlo method for helix and kink characterization in proteins. 53rd Annual Meeting of the Canadian Society of Biochemistry, Molecular and Cellular Biology, Banff, AB. (Poster) Student poster award & student travel award to D. Langelaan.

57.   Langelaan, D.N. and Rainey, J.K. (2010) Developing the N-terminus and first transmembrane segment of the GPCR APJ as a probe for ligand-receptor interactions. 53rdAnnual Meeting of the Canadian Society of Biochemistry, Molecular and Cellular Biology, Banff, AB. (Poster)

56.   Ngweniform, P. and Rainey, J.K. (2010) Biophysical characterization of interactions between the G-protein coupled receptor APJ and a fluorescently-tagged apelin analogue in micellar media. 53rd Annual Meeting of the Canadian Society of Biochemistry, Molecular and Cellular Biology, Banff, AB. (Poster)

55. Rainey, J.K. (2010) Correlating function to structure and dynamics in NHE1 using the �divide and conquer� approach. Na+/H+ Exchangers Satellite Symposium, 53rd Annual Meeting of the Canadian Society of Biochemistry, Molecular and Cellular Biology, Banff, AB. (Invited oral presentation)

54.   Banks, A.W. and Rainey, J.K. (2010) Correlating triple-helical collagen monomer morphology to local thermodynamic properties. 9th Chemical Biophysics Symposium, Toronto, ON. (Contributed poster; invited to give an oral presentation in Force & Fluorescence Microscopy Workshop.) Student travel award to A. Banks.

53.   Langelaan, D.N. and Rainey, J.K. (2010) Structural and topological characterization of the N-terminus and first transmembrane segment of the G-protein coupled receptor APJ. 9th Chemical Biophysics Symposium, Toronto, ON.

52.   Tremblay, M-L., Banks, A.W. and Rainey, J.K. (2010) Protein secondary structure prediction by secondary chemical shifts and its relation to solvent environment. 9th Chemical Biophysics Symposium, Toronto, ON. (Oral presentation) Student travel award to M. Tremblay.

51. Langelaan, D.N. & Rainey, J.K. (2010) Structure, dynamics and topology of the N-terminus and first transmembrane segment of APJ. 54th Annual Meeting of the Biophysical Society, San Francisco, CA. (Poster) Student travel award to D. Langelaan.

50. Rainey, J.K. (2009) Spectacular sensitivity for biomolecular NMR on the new NMR-3/NRC-IMB 700 MHz spectrometer. Nuclear Magnetic Resonance Research Resource (NMR-3) 4th Annual NMR Spectroscopy Workshop, Halifax, NS. (Oral presentation)

49. Langelaan, D.N. and Rainey, J.K. (2009) Apelin-micelle interactions are headgroup dependent. Nuclear Magnetic Resonance Research Resource 4th Annual NMR Spectroscopy Workshop, Halifax, NS. (Poster)

48.   Reddy, T., Sykes, B.D. and Rainey, J.K. (2009) 15N backbone NMR relaxation studies of transmembrane segment VII of the Na+/H+ exchanger isoform 1. Nuclear Magnetic Resonance Research Resource 4th Annual NMR Spectroscopy Workshop, Halifax, NS. (Poster)

47.   Langelaan, D.N. & Rainey, J.K. (2009) Dramatic micelle binding induced conformational change in the bioactive peptide apelin. 22nd MOOT Mini NMR Symposium, Ottawa, ON. (Oral presentation)

46.   Langelaan, D.N., Bebbington E.M. and Rainey, J.K. (2009) The GPCR ligand apelin attains specific structure when interacting with micelle surfaces. 52nd Annual Meeting of the Canadian Society for Biochemistry, Molecular and Cell Biology, Niagara-on-the-Lake, ON. (Poster)

45.   Reddy, T., Sykes B.D. and Rainey, J.K. (2009) Correlating structure, dynamics, and function in transmembrane segment VII of the Na+/H+ exchanger isoform 1. 52nd Annual Meeting of the Canadian Society for Biochemistry, Molecular and Cell Biology, Niagara-on-the-Lake, ON. (Poster)

44.   Tremblay, M-L., Banks, A.W. and Rainey, J.K. (2009) Effects of solvent dielectric on 1H and 13C NMR random coil chemical shifts. 52nd Annual Meeting of the Canadian Society for Biochemistry, Molecular and Cell Biology, Niagara-on-the-Lake, ON. (Poster) Student travel award to M. Tremblay.

43.  Langelaan, D.N. and Rainey, J.K. (2009) Headgroup dependent interactions of the peptide apelin with micelle surfaces. Chemical Biophysics Symposium, Toronto, ON. (Oral presentation) Student travel award to D. Langelaan.

42.  Reddy, T., Sykes, B.D. and Rainey, J.K. (2009) Model-free analysis and reduced spectral density mapping of a micelle-solubilized peptide using 15N relaxation data. Chemical Biophysics Symposium, Toronto, ON. (Poster)

41.  Tremblay, M-L. and Rainey, J.K. (2009) Effects of solvent dielectric on 1H and 13C random coil chemical shifts. Chemical Biophysics Symposium, Toronto, ON. (Poster)

40.  Langelaan, D.N. and Rainey, J.K. (2008) A structural study of the peptide apelin and interactions with its receptor APJ. Atlantic Region Magnetic Resonance Centre NMR Spectroscopy Workshop, Halifax, NS. (Oral presentation)

39.   Reddy, T., Sykes, B.D., Fligel, L. and Rainey, J.K. (2008) Structure & dynamics of Na+/H+ exchanger isoform 1 (NHE1) transmembrane segments. Atlantic Region Magnetic Resonance Centre NMR Spectroscopy Workshop, Halifax, NS. (Oral presentation)

38.   Langelaan, D.N. and Rainey, J.K. (2008) NMR spectroscopy shows definite structuring to a �random-coil� peptide. International Conference on Magnetic Resonance of Biological Systems. San Diego, CA. (Poster)

37.   Reddy, T.,  Ding, J., Li, X., Sykes, B.D., Fliegel, L. and Rainey, J.K. (2008) Correlating structure and function for transmembrane segment IX of the human Na+/H+ exchanger isoform 1. International Conference on Magnetic Resonance of Biological Systems. San Diego, CA. (Poster)

36.   Rainey, J.K., Langelaan, D.N. and Reddy, T. (2008) Detecting and dealing with conformational sampling in peptides. International Conference on Magnetic Resonance of Biological Systems. San Diego, CA. (Poster)

35.   Melong, J.C., Langelaan, D.N., Grant, M.A. and Rainey, J.K. (2008) Synthesis, purification and structural analysis of an apelin analog peptide using NMR characterization. Rising Stars of Research. Vancouver, BC. (Poster) Travel award to J. Melong.

34.   Lemieux, M.J., Lamoureux, J., Mak, M., Fischer, S., Rainey, J.K., Reddy, T. and James, M. (2008) Structural and functional studies of the Rhomboid intramembrane peptidase from H. influenzae. 22nd Symposium of the Protein Society, San Diego, CA. (Oral presentation)

33.   Langelaan, D.N., Bebbington, E.M., Reddy, T. and Rainey, J.K. (2008) Structure in a random-coil peptide: the apelin story. APICS/ChemCon, Halifax, NS. (Poster)

32.   Reddy, T.,  Ding, J., Li, X., Sykes, B.D., Fliegel, L. and Rainey, J.K. (2008) Structure and topology of transmembrane segment IX of the human Na+/H+ exchanger isoform 1. APICS/ChemCon, Halifax, NS. (Oral presentation)

31.   Langelaan, D.N., Bebbington, E.M., Reddy, T. and Rainey, J.K. (2008) NMR, circular dichroism & fluorescence spectroscopy provide insight into the random-coil nature of apelin peptides. Chemical Biophysics Symposium, Toronto, ON. (Poster)

30.   Reddy, T.,  Ding, J., Sykes, B.D., Fliegel, L. and Rainey, J.K. (2008) Structural and functional characterization of transmembrane segment IX of the Na+/H+ exchanger isoform 1. Chemical Biophysics Symposium, Toronto, ON. (Poster) Student travel award to T. Reddy.

29.   Langelaan, D.N., Bebbington, E.M., Reddy, T., Grant, M. and Rainey, J.K. (2008) Conformational sampling by apelin peptides gives functional insight. Joint 52nd Annual Meeting of the Biophysical Society & 16th IUPAB International Biophysics Congress, Long Beach, CA. (Poster)

28. Rainey, J.K. (2007)  Structural and biophysical characterization of membrane and extracellular matrix proteins. 6th Annual Canadian Institutes of Health Research New Principal Investigators Meeting, Jackson�s Point, ON. (Poster)

27. Langelaan, D.N., Bebbington, E.M. and Rainey, J.K. (2007) Solution-state NMR studies of apelin peptides. Atlantic Region Magnetic Resonance Centre NMR Spectroscopy Workshop, Halifax, NS. (Poster)

26. Reddy, T., Rainey, J.K. (2007) NMR of transmembrane peptides. Atlantic Region Magnetic Resonance Centre NMR Spectroscopy Workshop, Halifax, NS. (Poster)

25. Wieczorek, M. and Rainey, J.K. (2007) A computational analysis of kinked transmembrane segments. Chemical Biophysics Symposium, Toronto, ON. (Poster)

24. Rainey, J.K. (2007) Peptide structures - Diamonds in the rough? Chemical Biophysics Symposium, Toronto, ON. (Oral Presentation)

23. Rainey, J.K., Wieczorek, M., Fliegel, L. and Sykes, B.D. (2007) Identifying and understanding kinks in transmembrane helices. 51st Annual Meeting of the Biophysical Society, Baltimore, MD. (Poster)

22. Rainey, J.K. (2006) Structural studies of membrane proteins by solution- and solid-state NMR.  Atlantic Region Magnetic Resonance Centre NMR Spectroscopy Worskhop, Halifax, NS. (Oral presentation)

First author presentations by J.K. Rainey prior to arrival at Dalhousie

21. Rainey, J.K., Li, X., Liu, Y., Fliegel, L. and Sykes, B.D. (2006) Single transmembrane segments as a tool for structural study. 49th Annual Meeting of the Canadian Society for Biochemistry, Molecular and Cell Biology, Niagara on the Lake, ON. (Poster) Merck-Frosst Travel Award to J. Rainey.

20. Rainey, J.K., DeVries, J.S., and Sykes, B.D. (2005) A systematic consideration of NMR methods for membrane protein structural studies. Chemical Biophysics Symposium, Toronto, ON. (Oral presentation)

19. Rainey, J.K., DeVries, J.S., and Sykes, B.D. (2005) Comparing liquid and solid-state NMR structures of a transmembrane peptide. 229th American Chemical Society National Meeting, San Diego, CA. (Poster)

18. Rainey, J.K., DeVries, J.S., and Sykes, B.D. (2005) Optimizing conditions for solid-state NMR of membrane peptides. 49th Annual Meeting of the Biophysical Society, Long Beach, CA. (Poster)

17. Rainey, J.K., Lindhout, D.A., Li, X., Liu, Y., Fliegel, L., and Sykes, B.D. (2004) Membrane protein structure by dissection. Protein Engineering Network of Centres of Excellence Annual General Meeting, Montr�al, QC. (Poster)

16. Rainey, J.K. and Sykes, B.D. (2004) Trials, tribulations, and successes en route to membrane protein structure. Chemical Biophysics Symposium, Toronto, ON. (Poster)

15. Rainey, J.K. and Goh, M.C. (2003) Statistically derived reduced representations of amino acid side chains - a route to high-throughput structures? 3rd International Conference of the Canadian Proteomics Initiative, Vancouver, BC. (Poster)

14. Rainey, J.K. and Goh, M.C. (2003) Protein side-chain prediction with statistically derived reduced amino acid representations. Chemical Biophysics Symposium, Toronto, ON. (Poster)

13. Rainey, J.K., Paige, M.F., Wen, C.K., Lin, A.C. and Goh, M.C. (2002) Construction with collagen � Insight through atomic force microscopy. Microscopy & Microanalysis, Qu�bec, QC. (Poster)

12. Rainey, J.K., Wen, C.K. and Goh, M.C. (2002) Collagen-glycoprotein interactions leading to fibrillar self-assembly � A mechanistic investigation. Gordon Research Conference: Biopolymers, Newport, RI. (Poster)

11. Rainey, J.K. and Goh, M.C. (2002) Efficient and accurate protein modelling and visualization through minimal side-chain representation. Experimental Biology, New Orleans, LA. (Poster)

10. Rainey, J.K. and Goh, M.C. (2002) Statistically derived minimal side-chain conformations for accurate and functional protein representation. Chemical Biophysics Symposium, Toronto, ON. (Poster)

9. Rainey, J.K. and Goh, M.C. (2001) Minimal representations of  amino acid residue functionality and steric bulk from statistical analysis of culled high-resolution crystallographic data. Symposium on Chemical Physics, Waterloo, ON. (Poster)

8. Rainey, J.K., Manalo, J., and Goh, M.C. (2001) A minimal model of collagen � from model peptides and culled data-sets to a monomer structure. Gordon Research Conference: Bioinformatics � from Inference to Predictive Models, Tilton, NH. (Poster)

7. Rainey, J.K., Manalo, J., and Goh, M.C. (2001) Predicting collagen monomer structure and surface properties. 15th Protein Society Symposium, Philadelphia, PA. (Poster)

6. Rainey, J.K., Wen, C.K., Manalo, J. and Goh, M.C. (2001) Surface charge interactions and steric bulk in directed aggregation of collagen. 84th Canadian Society for Chemistry Conference & Exhibition, Montr�al, QC. (Oral presentation)

5. Rainey, J.K. and Goh, M.C. (2000) Influence of collagen monomer surface charge on aggregate morphology. 220th American Chemical Society National Meeting, Washington, DC. (Poster)

4. Rainey, J.K. and Goh, M.C. (2000) Correlating nuclear magnetic resonance spectroscopy and atomic force microscopy studies of segmental long spacing collagen crystallite structure. 14th Protein Society Symposium, San Diego, CA. (Poster)

3. Rainey, J.K., Paige, M.F., Lin, A.C., and Goh, M.C. (2000) Fibrous long spacing collagen polymorphism, ultrastructure and assembly. 2nd International Symposium on Advanced Biomaterials, Montr�al, QC. (Oral presentation)

2. Rainey, J.K., Paige, M.F., and Goh, M.C. (1999) Elucidation of fibrous long spacing collagen ultrastructure and assembly by atomic force microscopy and fluorescence. 82nd Canadian Society for Chemistry Conference & Exhibition, Toronto, ON. (Poster & Oral presentations)

1. Rainey, J.K., Paige, M.F., and Goh, M.C. (1998) Fibrous long spacing collagen ultrastructure and assembly elucidated by atomic force microscopy and fluorescence. 26th Southwestern Ontario Undergraduate Student Chemistry Conference, Toronto, ON. (Oral presentation)

Last Update: Nov 30, 2017